Background: Hyaline layer of Hopewell-Smith is a homogenous layer between the most external layer of dentin and the internal layer of acellular cementum. The development and functions of intermediate cementum are always controversial.
Aim: To analyze the morphological and histochemical traits of hyaline layer of Hopewell-Smith.
Materials and methods: Analysis of morphological traits were carried out in ground sections of 30 adult teeth with an age range of 20–50 years by polarized light microscopy. Histochemical traits of hyaline layer of Hopewell-Smith was done by using Periodic Acid Schiff and Alcian blue staining.
Results: Morphological traits such as thickness in different regions of root were found to show significant regional variation. Histochemical staining revealed clear demarcation between the dentin and cementum, hyaline layer proved to be a part of the cemental layer than dentin.
Conclusion: Findings such as regional variation in thickness have a remarkable impact on periodontal regeneration. Further correlates with electron microscopic studies will help newer treatment modalities for periodontal pathologies.
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