Aim and objective: The aim and objective of this study was to identify the minimum inhibitory concentration (MIC) of pure concentrated sesame seed powder require to inhibit growth of periodontal pathogens like Porphyromonas (P.) gingivalis (Pg), Prevotella (P.) intermedia (Pi), Fusobacterium (F) nucleatum (Fn), Tannerella (T.) forsythia (Tf). Materials and methods: Sesame seeds powder was obtained and minimum inhibitory concentration required to inhibit the growth of periodontal pathogens like P. gingivalis, P. intermedia, F. nucleatum, T. forsythia was determined by using the tube dilution method. Results: MIC of sesame seed for Pg showed sensitive at 3.12 mg/mL, Pi at 6.25 mg/mL, Fn at 3.12 mg/mL, Tf at 50 mg/mL. Fn was sensitive until 3.12 mg/mL showed resistance to further dilution by illuminating its MIC. Hence, MIC values were considered as Tf < Pi < Pg = Fn which sensitive at these concentration. Conclusion: Many studies in dentistry have shown its antibacterial efficacy on aerobic bacteria however, the present study findings conclude that the sesame seed powder exerts antimicrobial actions against major periodontal pathogens like Pg, Pi, Fn and Tf. So, further extensive in vivo studies are required to confirm the findings of the present study and explore therapeutic implications of sesame seed powder in the management of periodontal diseases. Clinical significance: The antibacterial efficacy of sesame seed powder used as local drug delivery as chip, gel at particular concentration which will help to prevent further progression of periodontal pocket after scaling and root planning such as reduction of periodontal pocket.