Aim: The aim of the present study was to compare the effects of different chelating agents on the microhardness of the most superficial layer of root canal lumen dentin.
Materials and methods: Forty-two extracted single-rooted teeth were instrumented, and the roots were longitudinally sectioned in a buccolingual direction to expose the entire root canal extension. The specimens were distributed into five groups according to the different chelating agents used: 15% ethylenediaminetetraacetic acid (EDTA) solution, 15% EDTA gel, 10% citric acid, 5% malic acid, and control [no irrigation (n=2)]. A standard volume of 50 ìL of each chelating agent was used for 5 minutes. Dentin microhardness was measured with a Vickers indenter under a 50 gm load and a 15 second dwell time. Data were analyzed statistically by one-way analysis of variance and post hoc multiple comparison test at 5% significance level.
Results: Ethylenediaminetetraacetic acid solution, EDTA gel, and citric acid had the greatest overall effect causing decrease in dentin microhardness without a significant difference (p>0.05) from each other. However, these chelating agents differed significantly with malic acid (p<0.05).
Conclusion: All tested chelating solutions reduced microhardness of the most superficial root canal dentin layer. Ethylenediaminetetraacetic acid and citric acid were the most efficient.
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