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VOLUME 15 , ISSUE 2 ( July-December, 2023 ) > List of Articles


Evaluation of Mechanical, Chemical, and Structural Properties of Leukocyte Platelet-rich Fibrin, and Advanced Platelet-rich Fibrin: An In Vitro Study

Trijanya Gowda, Narayan N Valavalkar, Syeda M Fatima

Keywords : Degradation, Modulus of elasticity, Platelet-rich fibrin, Scanning electron microscope

Citation Information : Gowda T, Valavalkar NN, Fatima SM. Evaluation of Mechanical, Chemical, and Structural Properties of Leukocyte Platelet-rich Fibrin, and Advanced Platelet-rich Fibrin: An In Vitro Study. CODS J Dent 2023; 15 (2):48-54.

DOI: 10.5005/jp-journals-10063-0152

License: CC BY-NC 4.0

Published Online: 17-05-2024

Copyright Statement:  Copyright © 2023; The Author(s).


Aim: This study was an attempt to evaluate the mechanical and chemical degradation properties of the fibrin architecture and to compare leukocyte–platelet-rich fibrin (L-PRF) and advanced PRF (A-PRF) based on these properties. Materials and methods: A total of 30 subjects, with 10 subjects for each test, were taken to evaluate the properties of the PRF membranes. The blood samples were immediately centrifuged using a tabletop centrifuge at 1,500 RPM for 14 minutes for A-PRF and 2,700 RPM for 12 minutes for L-PRF. A tensile test was performed using a universal testing machine. The in vitro degradation test of the prepared PRF clots was conducted by placing the PRF clots in 0.01% trypsin solution containing 2.5% glutaraldehyde in 0.1 M phosphate buffer solution for 24–48 hours. Scanning electron microscope (SEM) evaluation of the PRF clots was done under both low and high magnification. Results: The results showed a statistically significant increase in the tensile strength, modulus of elasticity, and toughness of L-PRF when compared with A-PRF. The degree of degradation was statistically decreased in L-PRF when compared with A-PRF. On SEM analysis, L-PRF showed leukocytes and platelets were clumped with dense matrix formation with increased interfibrous space, while in A-PRF, leukocytes and platelets were well distributed along the entire surface of the membrane with decreased interfibrous space. Conclusion: Results from the present study indicate that L-PRF through high-speed centrifugation has enhanced mechanical and structural properties, which yields a membrane with characteristics that are desirable for guided tissue regeneration (GTR) procedures.

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