CODS - Journal of Dentistry

Register      Login

VOLUME 13 , ISSUE 2 ( July-December, 2021 ) > List of Articles


An In Vitro Comparison of 5% Potassium Nitrate and 8% Proargin Desensitizing Toothpaste: A Scanning Electron Microscopy Study

E Neethu Krishna, Shobha Prakash

Keywords : Dentin hypersensitivity, Oral health, Oral hygiene, Scanning electron microscope

Citation Information : Krishna EN, Prakash S. An In Vitro Comparison of 5% Potassium Nitrate and 8% Proargin Desensitizing Toothpaste: A Scanning Electron Microscopy Study. CODS J Dent 2021; 13 (2):47-50.

DOI: 10.5005/jp-journals-10063-0123

License: CC BY-NC 4.0

Published Online: 01-06-2022

Copyright Statement:  Copyright © 2021; The Author(s).


Aim: This study is an in vitro comparison of 5% potassium nitrate and 8% arginine that have been applied in the treatment of dentinal hypersensitivity and scanning electron microscopy (SEM) is used to evaluate and compare changes in tubule dimensions after the application of the desensitizing agents. Materials and methods: A total of 24 disk-shaped dentin specimens were dissected from extracted premolars and molars. Dentin disks of 1 mm were obtained with the help of diamond disks. Eight specimens were randomly selected and allocated to the evaluation groups I (control group), II (5% potassium nitrate), and III (proargin). Each treatment group dentin disk specimen was treated with the respective toothpaste for 2 minutes for a period of 14 days. Each disk was subjected to a pre- and post-treatment SEM analysis to evaluate the changes occurring in the dentinal tubules. Results: The desensitizing toothpastes showed both complete and partial occlusion of dentinal tubules. There was a statistical significant difference between groups II and III, groups III and I, and groups I and II (p-value < 0.005). Conclusion: It was concluded that the two desensitizing agents were effective in the dentin tubule occlusion. In addition, efficacy of proargin toothpaste was greater compared to the 5% potassium nitrate containing toothpaste.

  1. Kulal R, Jayanti I, Sambashivaiah S, et al. An in-vitro comparison of nano hydroxyapatite, novamin and proargin desensitizing toothpastes—a SEM study. J Clin Diagn Res 2016;10(10):ZC51–ZC54. DOI: 10.7860/JCDR/2016/18991.8649
  2. Petrou I, Heu R, Stranick M, et al. A breakthrough therapy for dentin hypersensitivity: how dental products containing 8% arginine and calcium carbonate work to deliver effective relief of sensitive teeth. J Clin Dent 2009;20(1):23–31. PMID: 19489189.
  3. Jena A, Kala S, Shashirekha G. Comparing the effectiveness of four desensitizing toothpastes on dentinal tubule occlusion: a scanning electron microscope analysis. J Conserv Dent 2017;20(4):269–272. DOI: 10.4103/JCD.JCD_34_17
  4. James JM, Puranik MP, Sowmya KR. Dentinal tubule occluding effect of potassium nitrate in varied forms, frequencies and duration: an in vitro SEM analysis. J Clin Diagn Res 2017;11(8):ZC06–ZC08. DOI: 10.7860/JCDR/2017/26442.10340
  5. Schellenberg U, Krey G, Bosshardt D, et al. Numerical density of dentinal tubules at the pulpal wall of human permanent premolars and third molars. J Endod 1992;18(3):104–109. DOI: 10.1016/S0099-2399(06)81308-7
  6. Absy EG, Addy M, Adams D. Dentin hypersensitivity: the development and evaluation of a replica technique to study sensitive and non-sensitive cervical dentin. J Clin Periodontol 1989;16(3):190–195. DOI: 10.1111/j.1600-051X.1989.tb01639.x
  7. Pashley DH. Mechanisms of dentin sensitivity. Dent Clin North Am 1990;34(3):449–473. PMID: 19489189.
  8. Pereira JC, Martineli AC, Tung MS. Replica of human dentin treated with different desensitizing agents: a methodological SEM study in vitro. Braz Dent J 2002;13(2):75–85. DOI: 10.1590/S0103-64402002000200001
  9. Jain P, Vargas MA, Denehy GE, et al. Dentin desensitizing agents: SEM and X-ray microanalysis assessment. Am J Dent 1997;10(1):21–26. PMID: 19489189.
  10. Ahmed TR, Mordan NJ, Gilthorpe MS, et al. In vitro quantification of changes in human dentin tubule parameters using SEM and digital analysis. J Oral Rehabil 2005;32(8):589–597. DOI: 10.1111/j.1365-2842.2005.01473.x
  11. Reddy GV, Akula S, Malgikar S, et al. Comparative scanning electron microscope analysis of diode laser and desensitizing toothpastes for evaluation of efficacy of dentinal tubular occlusion. J Indian Soc Periodontol 2017;21(2):102–106. DOI: 10.4103/jisp.jisp_153_17
  12. Yoshiyama M, Masada J, Uchida A, et al. Scanning electron microscopic characterization of sensitive vs. insensitive human radicular dentin. J Dent Res 1989;68(11):1498–1502. DOI: 10.1177/00220345890680110601
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.