CODS Journal of Dentistry

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VOLUME 13 , ISSUE 1 ( January-June, 2021 ) > List of Articles

ORIGINAL RESEARCH

Hyaline Layer of Hopewell-Smith: A Morphometric and Histochemical Analysis

G Nimoshini, Ramya Ramadoss, R Swarnalakshmi, V Vasanthi, A Ramesh Kumar, Bose Divya

Keywords : Acellular cementum, Cementum, Hopewell-Smith layer, Hyaline layer, Intermediate cementum

Citation Information : Nimoshini G, Ramadoss R, Swarnalakshmi R, Vasanthi V, Kumar AR, Divya B. Hyaline Layer of Hopewell-Smith: A Morphometric and Histochemical Analysis. CODS J Dent 2021; 13 (1):3-5.

DOI: 10.5005/jp-journals-10063-0085

License: CC BY-NC 4.0

Published Online: 27-12-2021

Copyright Statement:  Copyright © 2021; The Author(s).


Abstract

Background: Hyaline layer of Hopewell-Smith is a homogenous layer between the most external layer of dentin and the internal layer of acellular cementum. The development and functions of intermediate cementum are always controversial. Aim: To analyze the morphological and histochemical traits of hyaline layer of Hopewell-Smith. Materials and methods: Analysis of morphological traits were carried out in ground sections of 30 adult teeth with an age range of 20–50 years by polarized light microscopy. Histochemical traits of hyaline layer of Hopewell-Smith was done by using Periodic Acid Schiff and Alcian blue staining. Results: Morphological traits such as thickness in different regions of root were found to show significant regional variation. Histochemical staining revealed clear demarcation between the dentin and cementum, hyaline layer proved to be a part of the cemental layer than dentin. Conclusion: Findings such as regional variation in thickness have a remarkable impact on periodontal regeneration. Further correlates with electron microscopic studies will help newer treatment modalities for periodontal pathologies.


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  1. Gonclaves PF, Sallum EA, Sallum AW, et al. Dental cementum reviewed: development, structure, composition, regeneration and potential functions. Braz J Oral Sci 2005;4:651–658. DOI: 10.20396/bjos.v4i12.8641790
  2. Consolaro A, Renata B, Consolaro LA, et al. Methods for studying tooth root cementum by light microscopy. Int J Oral Sci 2012;4:119–128. DOI: 10.1038/ijos.2012.57
  3. Bosshardt DD, Selvig KA. Dental cementum: the dynamic tissue covering of the root. Periodontol 2000 1997;13:41–75. DOI: 10.1111/j.1600-0757.1997.tb00095.x
  4. Shukla D, Vinuth DP, Soumya S, et al Cemetum made more visual. J Forensic Odontostomatol 2012;30:29–37.
  5. Yamamoto T, Hasegawa T, Yamamoto T, et al. Histology of human cementum: its structure, function, and development. Jpn Dent Sci Rev 2016;52:63–74. DOI: 10.1016/j.jdsr.2016.04.002
  6. Xiong J, Gronthos S, Bartold PM. Role of the epithelial cell rests of Malassez in the development, maintenance and regeneration of periodontal ligament tissues. Periodontol 2000 2013;63:217–233. DOI: 10.1111/prd.12023
  7. Sucheta A, Spandana A, Sapna N, et al. Hertwig's epithelial root sheath: a panaromic view. Int J Appl Dent Sci 2017;3:21–25
  8. Slavkin HC, Boyde A. Cementum: an epithelial secretory product? J Dent Res 1974;53:157.
  9. Lindskog S. Formation of intermediate cementum. I: early mineralization of aprismatic enamel and intermediate cementum in monkey. J Craniofac Genet Dev Biol 1982;2:147–160.
  10. Lindskog S. Formation of intermediate cementum. II: a scanning electron microscopic study of the epithelial root sheath of Hertwig in monkey. J Craniofac Genet Dev Biol 1982;2:161–169.
  11. Nalbandian J, Frank RM. Electron microscopic study of the regeneration of cementum and periodontal connective tissue attachment in the cat. J Periodontal Res 1980;15:71–89. DOI: 10.1111/j.1600-0765.1980.tb00261.x
  12. Craig KR, Harrison JW. Wound healing following demineralization of resected root ends in periradicular surgery. J Endod 1993;19:339–347. DOI: 10.1016/S0099-2399(06)81360-9
  13. Boyde A, Jones SJ. Scanning electron microscopy of cementum and Sharpey fibre bone. Z Zellforsch Mikrosk Anat 1968;92:536–548. DOI: 10.1007/BF00336664
  14. Morris ML. The reattachment of human periodontal tissues following surgical detachment: a clinical and histological study. J Periodontol 1953;24:220–228. DOI: 10.1902/jop.1953.24.4.220
  15. Lindskog S, Blomlof L, Hammarstrom L. Repair of periodontal tissues in vivo and in vitro. J Clin Periodontol 1983;10:188–205. DOI: 10.1111/j.1600-051x.1983.tb02207.x
  16. Listgarten MA, Rosenberg MM. Histological comparison of periodontal wound healing in the beagle dog with and without citric acid conditioning. J Periodontal Res 1979;50:330–344.
  17. Cherian G. Harvesting cementum from root surface: a new paradigm in the study of cementum and the cemento-dentinal junction. J Adv Oral Res 2011;2(2):17–20. DOI: 10.1177/2229411220110203
  18. Esposito M, Grusovin MG, Papanikolaou N, et al. Enamel matrix derivative (Emdogain®) for periodontal tissue regeneration in intrabony defects. Aust Dent J 2010;55(1):101–104. DOI: 10.1111/j.1834-7819.2009.01186.x
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