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VOLUME 11 , ISSUE 2 ( July-December, 2019 ) > List of Articles
Mallanagouda B Patil, Anurag Bhatnagar, Shobha Prakash
Keywords : Chronic periodontitis, Periodontally healthy, Polymerase chain reaction,Archaea
Citation Information : Patil MB, Bhatnagar A, Prakash S. Prevalence of Methanobrevibacter oralis in Chronic Periodontitis Patients: A Pilot Study. CODS J Dent 2019; 11 (2):32-35.
License: CC BY-NC 4.0
Published Online: 16-10-2020
Copyright Statement: Copyright © 2019; Jaypee Brothers Medical Publishers (P) Ltd.
Background: Periodontitis is polymicrobial infection. Literature evidences supports the use of 16S ribosomal RNA ribotyping method to detect more than 100 noncultivable bacterial species present in subgingival microflora, concluding “plaque is composed of nonspecific opportunistic pathogens” that induce species like methanogenic archaea. Archaea are found in the oral cavity of human in the saliva, oral biofilms, endodontic lesions, and subgingival deep periodontal pockets of periodontitis. Chronic periodontitis has been linked to multiple members of the domain bacteria; however, not one member explains its role in the periodontitis. There are few studies that linked archaea to the subgingival biofilm of chronic periodontitis. The purpose of this study is to determine the prevalence of archaea in periodontally healthy and chronic periodontitis. Materials and methods: A total of 30 subjects (age range: 25–60 years), 15 subjects each in periodontally healthy and chronic periodontitis, participated in this study to find the prevalence of Methanobrevibacter (M.) oralis. Clinical parameters including probing pocket depth (PPD) and clinical attachment level (CAL) were recorded. The M. oralis was detected using the real-time polymerase chain reaction. Results: The prevalence of archaea in chronic periodontitis is 40% and in healthy subjects 6.7%. Conclusion: Chronic periodontitis showed more prevalence of archaea in periodontal pockets, which may suggest association with periodontitis.
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