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VOLUME 10 , ISSUE 1 ( January-June, 2018 ) > List of Articles

Original Article

Comparative Evaluation of Antimicrobial Activity of Quercetin against Endodontic Pathogens in Primary Molars

Shagun Sinha, Poornima Parameswarappa, Neena I Eregowda, Mallikarjun Kenchappa, Shilpa Sasalawad

Keywords : Antimicrobial, Chlorhexidine, Endodontic pathogen, Primary molars, Quercetin

Citation Information : Sinha S, Parameswarappa P, Eregowda NI, Kenchappa M, Sasalawad S. Comparative Evaluation of Antimicrobial Activity of Quercetin against Endodontic Pathogens in Primary Molars. CODS J Dent 2018; 10 (1):7-10.

DOI: 10.5005/jp-journals-10063-0042

License: CC BY-NC 4.0

Published Online: 01-04-2020

Copyright Statement:  Copyright © 2018; The Author(s).


Background: Endodontic irrigants have an important role in endodontic treatment of primary teeth, owing to their complex anatomy. Quercetin is a natural flavonoid that exhibits significant antimicrobial properties. Objective: To evaluate the antimicrobial activity of 0.2% chlorhexidine, 1% (w/v) quercetin, and 2% (w/v) quercetin against endodontic pathogens in primary molars. Materials and methods: A total of 15 patients of 4–7 years of age with nonvital carious primary molars were selected. A sample from the root canal was obtained using number 15 paper point. Antimicrobial activity was measured using the disk diffusion method, for the following solutions: group I: 0.2% chlorhexidine solution, group II: 1% w/v quercetin solution, and group III: 2% w/v quercetin solution. For each sample, microbial culture was done under the microaerophilic condition. After 24 hours, the maximum inhibition zone was recorded in millimeters. Results: The highest inhibition was shown by group I, 13.13 ± 0.74, followed by group III, 12.00 ± 0.65, and the lowest in group II, i.e., 8.53 ± 0.63. On comparison between groups, statistically significant difference was noted. Conclusion: Maximum antimicrobial activity was shown by 0.2% chlorhexidine, followed by 2% quercetin that showed significant antimicrobial activity against endodontic pathogens in primary molars.

  1. Zehnder M. Root canal irrigants. J Endod 2006;32(5):389–398. DOI: 10.1016/j.joen.2005.09.014.
  2. Rana V, Baba SM, Pandey A. Bacteriology of infected deciduous root canal – a review. A PJSR 2009;2(2):45–48.
  3. Thomas AM, Chandra S, Chandra S, et al. Elimination of infection in pulectomized deciduous teeth: a short-term study using iodoform paste. J Endod 1994;20(5):233–235. DOI: 10.1016/S0099-2399(06)80284-0.
  4. Clarkson RM, Moule AJ. Sodium hypochlorite and its use as an endodontic irrigant. Aust Dent J 1998;43(4):250–256. DOI: 10.1111/j.1834-7819.1998.tb00173.x. Review.
  5. Khademi A, Usefian E, Feizianfard M. Tissue dissolving ability of several endodontic irrigants on bovine pulp tissue. Iran Endod J 2007;2(2):65–68.
  6. Majorie MC. Plant products as antimicrobial agents. Clin Microbiol Rev 1990;12(4):564–582.
  7. Vinothkumar TS, Rubin MI, Balaji L, et al. In vitro evaluation of five different herbal extracts as an antimicrobial endodontic irrigant using real-time quantitative polymerase chain reaction. J Conserv Dent 2013;16(2):167–170. DOI: 10.4103/0972-0707.108208.
  8. Middleton EJ. Effect of plant flavonoids on immune and inflammatory cell function. Adv Exp Med Biol 1998;439:175–182. DOI: 10.1007/978-1-4615-5335-9_13.
  9. Shu Y, Liu Y, Li L, et al. Antibacterial activity of quercetin on oral infectious pathogens. Afr J Microbiol Res 2011;5(30):5358–5361.
  10. Verma MK, Pandey RK, Khanna R, et al. The antimicrobial effectiveness of 25% propolis extract in root canal irrigation of primary teeth. J Indian Soc Pedod Prev Dent 2014;32(2):120–124. DOI: 10.4103/0970-4388.130786.
  11. Bhargava K, Kumar T, Aggarwal S, et al. Comparative evaluation of the antimicrobial efficacy of neem, green tea, triphala and sodium hypochlorite: an in vitro study. J Dent Res Rev 2015;2(1):13–16. DOI: 10.4103/2348-2915.154638.
  12. Shen Y, Stojicic S, Qian W, et al. The synergistic antimicrobial effect by mechanical agitation and two chlorhexidine preparations on biofilm bacteria. J Endod 2010;36(1):100–104. DOI: 10.1016/j.joen.2009. 09.018.
  13. Ruviére DB, Leonardo MR, da Silva LA, et al. Assessment of the microbiota in root canals of human primary teeth by checkerboard DNA-DNA hybridization. J Dent Child 2007;74(2):118–123.
  14. Sundqvist G, Figdor D, Persson S, et al. Microbiologic analysis of teeth with failed endodontic treatment and the outcome conservative treatment. Oral Surg Oral Med Oral Pathol 1998;85(1):86–93. DOI: 10.1016/S1079-2104(98)90404-8.
  15. Bulacio Mde L, Cangemi R, Cecilia M, et al. In vitro antibacterial effect of different irrigating solutions on Enterococcus faecalis. Acta Odontol Latinoam 2006;19(2):75–80.
  16. Ferraz CC, Gomes BP, Zaia AA, et al. Comparative study of the antimicrobial efficacy of chlorhexidine gel, chlorhexidine solution and sodium hypochlorite as endodontic irrigants. Braz Dent J 2007;18(4):294–298. DOI: 10.1590/S0103-64402007000400004.
  17. Gomes BPFA, Souza SFC, Ferraz CCR, et al. Effectiveness of 2% chlorhexidine gel and calcium hydroxide against Enterococcus faecalis in bovine root dentine in vitro. Int Endod J 2003;36(4):267–275. DOI: 10.1046/j.1365-2591.2003.00634.x.
  18. Li M, Xu Z. Quercetin in a lotus leaves extract may be responsible for antibacterial activity. Arch Pharm Res 2008;31(5):640–644. DOI: 10.1007/s12272-001-1206-5.
  19. Patra JK, Kim ES, Oh K, et al. Antibacterial effect of crude extract and metabolites of Phytolacca americana on pathogens responsible for periodontal inflammatory diseases and dental caries. BMC Complement Altern Med 2014;14:343. DOI: 10.1186/1472-6882-14-343.
  20. Helena T-C Silvia, José SM, Evandro W, et al. Antimicrobial activity of flavonoids and steroids isolated from two Chromolaena species. Rev Bras Cienc Farm 2003;39(4):403–408. DOI: 10.1590/S1516-93322003000400007.
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